October 2012 – B
|Ghiglione, et al., (2012).|
A study sheds light on the diversity found in the waters from the Arctic and Southern oceans. Researchers tested a number of samples from different depths (surface or deep water), locations (coastal or open sea) and time (winter or spring) in both polar water masses , as well as some samples from lower latitudes. It was found that most the microbial OTUs from the Arctic and Southern oceans are unique to each place, while 15% of the OTUs where shared.
|Carlson, et al., (2012).|
The generation of fusion proteins comprised by a DNA binding domain and a FokI zinc finger endonuclase developed recently in novel and site specific genome engineering techniques. Interestingly, the TALEN (Transcription activator-like effector nucleases) technique can be rationally designed to target specific sequences more easily than a technique based on engineered zinc fingers. Now, knockout livestock was successfully generated through TALEN mediated gene knockout in fibroblasts and cloning.
|Rostovksaya, et al., (2012).|
A recent study reports that BACs can be employed to generate transgenic human ES cells with large constructs (more than 19 – 25 kb) using transposition mechanisms. These results provide evidence on BACs utility despite the concerns on their physical stability.