February 2013 – A
|From Saphiro et al., (2013).|
|From Chappell, Jensen and Freemont, (2013).|
Cell-free characterization of regulatory elements
Researchers from the Centre for Synthetic Biology and Innovation at Imperial College describe a completely in vitro method for the characterization of genetic regulatory elements (constitutive promoters, RBS and inducible promoters). In their publication, they validate this method and show how it is correlated with in vivo measurements. Of remark is that the in vitro procedure can be performed in 5 h and may allow for faster annotation of libraries and prototyping standard biological parts.